Laboratory implications of incorrectly taken blood samples for antibiotic assay

Laboratory implications of incorrectly taken blood samples for antibiotic assay.

Assay of fibrinogen in small blood samples.

T IS DIFFICULT to perform accurate fibrinogen assays on small samples of blood. A significant fraction of the plasma is lost when it is separated from the cells in 0.1or 0.2-mi. samples of blood and the plasma is diluted to a variable, usually undetermined, extent by the addition of anticoagulant. This can be minimized by using heparin dried in capillary tubes, but heparin interferes with subse...

Laboratory assay reproducibility of serum estrogens in umbilical cord blood samples.

We evaluated the reproducibility of laboratory assays for umbilical cord blood estrogen levels and its implications on sample size estimation. Specifically, we examined correlation between duplicate measurements of the same blood samples and estimated the relative contribution of variability due to study subject and assay batch to the overall variation in measured hormone levels. Cord blood was...

Acidic citrate stabilizes blood samples for assay of total homocysteine.

min were as follows: At 70 MPa (10 000 lb./in.), no pressure effect was evident; ;10% of maximal binding was observed at 140 MPa, 20% at 210 MPa, 50% at 280 MPa, and 80% at 420 MPa. Preincubation of the anti-p24 antibody alone (without the p24 antigen) at 420 MPa for 10 min did not result in any discernible decrease in absorbance, indicating that pressures as great as 420 MPa did not affect the...

Direct enzymatic assay for %HbA1c in human whole blood samples.

OBJECTIVES Development and validation of a direct enzymatic HbA1c assay that utilizes a single channel on chemistry auto-analyzers without the need to run separate glycated hemoglobin and total hemoglobin assays. DESIGN AND METHODS An enzyme based single channel assay was developed to measure %HbA1c in human whole blood samples. The performance characteristics of the Diazyme Direct Enzymatic ...